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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in several tumors, which leads to chromosome instability, and therefore could lead to tumorigenesis. This protein was located to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important position in marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been explained. Two added splice variants had been claimed but thorough nucleotide sequence info is not really nevertheless accessible.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide normally incorporates the epitope recognized with the antibody.
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This information outlines additional possible optimization of mobile and immunoassay parameters to make sure the very best benefits are...
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This manual outlines additional achievable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Goal Details Cyclin E belongs for the highly conserved cyclin household, whose associates are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression more info and degradation patterns which add on the temporal coordination of each mitotic event. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is required for cell cycle G1/S transition. This protein accumulates in the G1-S section boundary which is degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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